Preface
Introduction
1 Sense Cosuppression in Plants:Past,Present,and Future
2 Transgene Cosuppression in Animals
3 Regulation of the Genome by Double-stranded RNA
4 RNAi in Caenorhabditis elegans
5 Small RNAs,Big Biology:Biochemical Studies of RNA Interfernece
6 shRNA-mediated Silencing of Mammalian Gene Expression
7 Transposable Elements,RNA Interference,and the Origin of Heterochromatin
8 The Ribonuclease III Superfamily:Forms and Functions in RNA
9 RNA-dependent RNA Polymerase in Gene Silencing
10 Structure and Function of Heterochromatin:Implications for Epigenetic Gene Silencing and Genome Organization
11 Total Interference:Genome-wide RNAi Screens in C.elegans
12 PTGS Approaches to Large-scale Functional Genomics in Plants
13 Mammalian RNA Interference
14 RNAi in Avian Embryos
15 Guide to RNAi in Mouse Oocytes and Preimplantation Embryos
16 RNAi Technologies in Drosophila Cell Culture
17 RNAi Applications in Drosophila melanogaster
18 RNA Interference in Trypanosoma brucei and Other Nonclassical Model Organisms
Appendices
1 Cautions
2 Suppliers
Index
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