Preface
Purifying Proteins for Proteomics Companion Web Site
Section Ⅰ:Preparing Proteins for Proteomic Analysis
1 Role of Separation Science in Proteomics
2 Strategies for Purifying Proteins
Section Ⅱ:Chromatographic Methods for Preparing Proteins for Proteomics
3 Introduction to Chromatographic Methods for Protein and Peptide Purification
4 Multidimensional Chromatography of intact Proteins
5 High-throughput Screening of Soluble Recombinant Proteins
6 lon-exchange Chromatography
7 Size-exclusion Chromatography
8 Role of Reversed-phase High-performance Liquid Chromatography in Protein Isolation and Purification
9 Hydrophobic Interaction Chromatography
1 Affinity and Immunoaffinity Chromatography
11 Dye-Ligand Affinity Chromatography in Proteomic Research
12 Immobilized Metal-ion Affinity Chromatography
13 Protein Chromatography on Hydroxyapatite
14 Chromatofocusing
Section Ⅲ:Electrophoretic Methods for Preparing Proteins for Proteomics
15 Introduction to Strategies Based on Electrophoresis for Separating Proteins
16 Separation of Proteins Using Two-dimensional Gel Electrophoresis
17 Sample Preparation for High-resolution Two-dimensional Electrophoresis by Isoelectric Fractionation in a MCE
18 An Electrophoresis Method for Purifying Proteins:The Gradiflow BF4 Instrument
19 Protein Purification Using Free-flow Electrophoresis
Section Ⅳ:Analysis of Purified Proteins Prior to Structural Proteomics
2 Introduction to Methods for Characterizing Purified Proteins
21 Characterization of Proteins by Mass Spectrometry
22 Analytical Ultracentrifugation:Protein Size,Conformation,and Interactions
23 Measuring the Conformational Stability of a Protein
24 Combining Surface Plasmon Resonance with Mass Spectrometry:ldentifying Binding Partners and Characterizing Interactions
25 Analysis of Carbohydrate from Glycoproteins
Appendices
1.An Overview of Protein Structure
2.Measuring the Concentration of Proteins
3.Concentrating Solutions of Proteins
4.Stabilization of Proteins for Storage
5.One-dimensional Polyacrylamide Gel Electrophoresis
6.Troubleshooting Guides
7.Cautions
Index
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