Preface
1.Developmental Genetics and Embryology of the Mouse:Past,Present,and Future
2.Summary of Mouse Development
3.Production of Transgenic and Chimeric Mice:General Issues
4.Recovery and In Vitro Culture of Preimplantation-stage Embryos
5.Isolation,Culture,and Manipulation of Postimplantation Embryos
6.Surgical Procedures
7.Production of Transgenic Mice
8.Isolation and Culture of Blastocyst-derived Stem Cell Lines
9.Bector Designs for Embryonic Stem Cell-based Transgenesis and Genome Alterations
1.Introduction of Foreign DNA into Embryonic Stem Cells
11.Production of Chimeras
12.Detection and Analysis of Mouse Genome Alterations and Specific Sequences
13.Parthenogenesis,Pronuclear Transfer,and Mouse Cloning
14.Assisted Reproduction:Ovary Transplantation,In Vitro Fertilization,Artificial Insemination,and Intracytoplasmic Sperm Injection
15.Cryopreservation,Rederivation,and Transport of Mice
16.Techniques for Visualizing Gene Products,Cells,Tissues,and Organ Systems
17.Setting Up a Micromanipulation Lab
Appendix 1:Buffers and Solutions
Appendix 2:Cautions
Appendix 3:Suppliers
Index
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