Experiment 1
Preparation of Plasmid DNA
Experiment 2
Restriction Enzyme Digestion and Agarose Gel Electrophoresis
Experiment 3
Polymerase Chain Reaction（PCR）
Experiment 4
DNA Purification and Construction of Recombinant DNA
Experiment 5
Preparation of Competent E.coli Cells
Experiment 6
Transformation of Recombinant DNA
Experiment 7
Identification and Characterization of Positive Clones
Experiment 8
Isolation ofTotal RNA from Animal Tissues
Experiment 9
Molecular Hybridization
Experiment 10
Reverse Transcription—Polymerase Chain Reaction（RT-PCR） and TA-cloning
Experiment 11
Expression and Purification of a Fusion Protein from E.coli
Experiment 12
Electrophoretic Mobility Gel Shift Assay（EMSA）to Detect DNA—Protein Interactions
Appendix I
Brief Introduction to the NCBI Database
Appendix II
Vector Information
Appendix III
Instructions on How to Use the Equipment
References
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